The following options will make a huge difference: Any of the above could conceivably be crossdressers.
Any of them could have one or more transgender people or bifauxnen as potential love interests.
If you have decided to create an adult game, then you need to decide how sex will be incorporated into the story or the gameplay.
You can include hardcore scenes at important moments.
Do not talk about your awesome plans until your project is almost finished.
You could also make them bisexual if you want love interests of both genders, but don't put too many options or it can be overwhelming.
Unless you are making a story which is the equivalent of "gen" Fan Fic, you will have a main character who falls in love with one or more people, or vice versa.
" Remember, it could get worse — if your story involves horror/dark themes, and if your main character is doing horrible things, then your audience may want to see themselves as anyone but that character.
Note that Featureless Protagonist is exception to this.